PLGA-Rhodamine B (PLGA-RhB), MW 10K is a high-molecular-weight, red fluorescent labeled biodegradable PLGA copolymer covalently conjugated with Rhodamine B (RhB) fluorophore. Featuring enhanced structural rigidity, excellent morphological stability and prolonged hydrolytic degradation performance, this fluorescent polymer delivers bright, stable red fluorescence for long-cycle real-time optical tracing, in vivo biodistribution analysis and long-term nanoparticle visualization. It supports fully tunable PLGA compositions and custom molecular weight preparation, serving as a high-performance fluorescent tracking material for long-term sustained drug delivery and advanced biomedical imaging research.
Basic Specifications
Alternative Name: PLGA-RhB, Rhodamine B-PLGA
PLGA Composition: 50:50, 65:35, 75:25, 85:15, Custom
Molecular Weight: Custom
Fluorescent Label: Rhodamine B (RhB)
Excitation Wavelength: ~554 nm
Emission Wavelength: ~576 nm
Appearance: Pink to light red solid
Key Features and Benefits
Stable Covalent Rhodamine B Labeling: Rhodamine B fluorophore is stably covalently bonded to the terminal of PLGA polymer chains, effectively preventing fluorophore shedding, free dye leakage and non-specific background interference. The polymer maintains bright, consistent red fluorescence with low noise throughout long-term polymer degradation and in vivo circulation processes, ensuring high-reliability and repeatable long-cycle fluorescent tracing experimental results.
Fully Tunable PLGA Composition: Covers all mainstream lactide-glycolide ratios including 50:50, 65:35, 75:25, 85:15, and supports personalized custom synthesis. Precise ratio modulation flexibly adjusts polymer hydrophilicity, degradation kinetics and particle structural properties, fully adapting to various long-cycle sustained-release formulations and advanced long-term biomedical imaging scenarios.
High-Stability 10K Molecular Weight Performance: The 10K high molecular weight forms dense polymer chain entanglement, significantly improving structural rigidity, morphological stability and anti-deformation capability compared with medium and low-MW PLGA. It exhibits uniform and prolonged hydrolytic degradation behavior, effectively avoiding nanoparticle structural collapse, fatigue damage and premature drug release, perfectly suitable for preparing high-stability fluorescent nanoparticles and microspheres for long-cycle tracing research.
Excellent Biocompatibility & Low Cytotoxicity: Possesses superior physiological biocompatibility and biosafety. The PLGA backbone gradually and uniformly hydrolyzes into non-toxic endogenous lactic acid and glycolic acid in physiological environments. The covalently immobilized RhB fluorophore induces no obvious cellular toxicity or inflammatory response, fully meeting safety standards for long-term in vitro cell imaging and prolonged in vivo biological tracing applications.
High Signal Anti-Interference Ability: The red fluorescence signal of Rhodamine B falls in the long-wavelength optical window, which effectively avoids autofluorescence interference from biological tissues and culture media. It delivers a high signal-to-noise ratio and excellent imaging resolution, enabling accurate, intuitive visualization of nanoparticle cellular uptake, intracellular trafficking, tissue distribution and long-term in vivo accumulation behavior.
Applications
Long-Cycle Fluorescent Particle Tracing: Widely applied for real-time fluorescent monitoring and dynamic behavior analysis of high-stability PLGA nanoparticles, microspheres and functional hydrogel carriers, supporting long-cycle research on particle internalization, intracellular transport and in vivo tissue distribution rules.
Advanced Long-Term Biomedical Optical Imaging: Ideal for long-duration in vitro cell fluorescence imaging, in vivo tissue section tracing and biodistribution & metabolism evaluation of sustained-release drug delivery systems, providing intuitive optical data support for long-acting formulation optimization and delivery mechanism exploration.
Long-Cycle Sustained-Release Formulation Verification: Used for visual tracking of long-term polymer degradation and sustained drug release behavior, enabling researchers to intuitively evaluate the structural stability, release uniformity and long-term efficacy of prolonged sustained-release formulations.
Durable Biomaterial Distribution & Metabolism Analysis: Serves as a high-stability fluorescent marker for long-cycle degradable tissue engineering scaffolds and biomedical carriers, supporting prolonged in vivo material retention, metabolism process and biocompatibility evaluation research.
Storage and Handling
Storage: Store at –20 °C in a sealed, dry, light-proof and moisture-proof container to prevent fluorescence quenching and polymer hydrolysis, effectively protecting long-term fluorescent activity and polymer structural integrity.
Handling: Avoid prolonged strong light irradiation, high-temperature environment and repeated freeze-thaw cycles to prevent RhB fluorescence attenuation and polymer performance degradation.
PLGA-Rhodamine B (PLGA-RhB), MW 10K integrates high-stability long-cycle fluorescent tracing performance, fully tunable PLGA composition and prolonged degradability, serving as a high-performance fluorescent biodegradable polymer for long-term nanoparticle tracing, advanced biomedical optical imaging and prolonged sustained-release formulation visualization research.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.