PLGA-Rhodamine B (PLGA-RhB), MW 5K is a medium-molecular-weight, red fluorescent labeled biodegradable PLGA copolymer covalently conjugated with Rhodamine B (RhB) fluorophore. Featuring moderate structural rigidity, balanced solubility and steady medium-term degradability, this fluorescent polymer provides bright, stable red fluorescence for real-time optical tracing, biodistribution analysis and in vitro/in vivo nanoparticle visualization. It supports fully tunable PLGA compositions and custom molecular weight preparation, serving as a standard fluorescent tracking material for routine sustained drug delivery and biomedical imaging research.
Basic Specifications
Alternative Name: PLGA-RhB, Rhodamine B-PLGA
PLGA Composition: 50:50, 65:35, 75:25, 85:15, Custom
Molecular Weight: Custom
Fluorescent Label: Rhodamine B (RhB)
Excitation Wavelength: ~554 nm
Emission Wavelength: ~576 nm
Appearance: Pink to light red solid
Key Features and Benefits
Stable Covalent Rhodamine B Labeling: Rhodamine B fluorophore is covalently bonded to the PLGA polymer terminal, effectively avoiding fluorophore shedding, leakage and free dye interference. The polymer exhibits bright and stable red fluorescence with low background noise, maintaining consistent optical signal intensity during polymer degradation and particle circulation, ensuring reliable and repeatable fluorescent tracing results.
Fully Tunable PLGA Composition: Covers mainstream lactide-glycolide ratios including 50:50, 65:35, 75:25, 85:15 and supports custom composition synthesis. Precise ratio adjustment flexibly regulates polymer hydrophilicity, degradation rate and particle morphology, perfectly matching different medium-cycle sustained-release requirements and diverse biomedical imaging scenarios.
Balanced Medium-MW Comprehensive Performance: The customized 5K-level medium molecular weight achieves an optimal balance of excellent organic solubility and structural stability. Compared with low-molecular PLGA, it delivers higher morphological rigidity and uniform degradation behavior, effectively preventing burst release and structural failure, suitable for preparing stable fluorescent nanoparticles and microspheres for medium-term tracing research.
Excellent Biocompatibility & Low Cytotoxicity: Possesses superior physiological compatibility and low biological toxicity. The PLGA backbone gradually hydrolyzes into non-toxic lactic acid and glycolic acid in physiological environments, and the covalently bound RhB fluorophore causes no significant inflammatory stimulation or cellular damage, fully meeting the safety requirements for in vitro cell imaging and medium-term in vivo biological tracing.
High Signal Anti-Interference Ability: Rhodamine B red fluorescence belongs to the long-wavelength optical range, which can effectively avoid the autofluorescence interference of biological tissues and culture media. It features high signal-to-noise ratio and strong imaging resolution, enabling accurate visualization of nanoparticle distribution, cellular uptake and in vivo accumulation behavior.
Applications
Medium-Term Fluorescent Particle Tracing: Widely used for real-time fluorescent tracking and behavior monitoring of PLGA nanoparticles, microspheres and hydrogel carriers, supporting medium-cycle analysis of particle cellular uptake, intracellular trafficking and tissue distribution rules.
Biomedical Optical Imaging Research: Ideal for in vitro cell fluorescence imaging, in vivo tissue section tracing and biodistribution evaluation of biodegradable drug delivery systems, providing intuitive optical evidence for formulation optimization and delivery mechanism research.
Medium-Cycle Sustained-Release Formulation Verification: Applied for visual monitoring of medium-term polymer degradation and sustained drug release behavior, helping researchers intuitively evaluate the stability and release performance of sustained-release formulations.
Biomaterial Distribution & Metabolism Analysis: Serves as a stable fluorescent marker for biodegradable tissue engineering scaffolds and carrier materials, supporting medium-term in vivo material metabolism, retention and biocompatibility evaluation.
Storage and Handling
Storage: Store at –20 °C in a sealed, dry, light-proof and moisture-proof container to prevent fluorescence quenching and polymer hydrolysis, effectively protecting long-term fluorescent activity and structural integrity.
Handling: Avoid prolonged strong light irradiation, high-temperature environment and repeated freeze-thaw cycles to prevent RhB fluorescence attenuation and polymer performance degradation.
PLGA-Rhodamine B (PLGA-RhB), MW 5K integrates stable red fluorescent tracing performance, tunable PLGA composition and balanced medium-term degradability, serving as a standard high-sensitivity fluorescent biodegradable polymer for medium-cycle nanoparticle tracing, biomedical optical imaging and sustained-release formulation visualization research.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.