Methyltetrazine-PEG5-NHS combines a reactive methyltetrazine group, hydrophilic PEG5 spacer, and amine-reactive NHS ester in one bifunctional linker and specifically supports sequential bioconjugation, bioorthogonal click chemistry, molecular labeling, and functional biomaterial research.
Key Features and Benefits
- Bifunctional Linker: Combines methyltetrazine and NHS ester functionalities for sequential conjugation and molecular functionalization.
- Methyltetrazine Functionality: Provides a reactive tetrazine group for rapid bioorthogonal ligation with compatible strained dienophiles.
- PEG5 Spacer: The hydrophilic PEG5 chain specifically provides flexible spacing between the two reactive functional groups.
- NHS Ester Functionality: Reacts with primary amines on proteins, peptides, and other amine-containing molecules.
- Amine-Reactive Coupling: NHS ester chemistry specifically supports convenient attachment to compatible amino-functionalized molecules and materials.
- IEDDA Click Chemistry: Methyltetrazine specifically supports inverse-electron-demand Diels–Alder reactions with TCO and other compatible strained dienophiles.
- Copper-Free Conjugation: Tetrazine ligation proceeds without a copper catalyst, supporting metal-free bioorthogonal labeling strategies.
- Sequential Functionalization: NHS ester coupling can introduce the tetrazine functionality before subsequent bioorthogonal click reactions.
Applications
- Bioconjugation: Functionalization of proteins, peptides, ligands, and other primary amine-containing biomolecules.
- Tetrazine Click Chemistry: Supports rapid IEDDA conjugation with compatible TCO and strained dienophile-functionalized molecules.
- Protein Labeling: NHS ester functionality enables modification of accessible primary amines on proteins.
- Peptide Modification: Useful for introducing tetrazine functionality into compatible amine-containing peptides.
- Molecular Labeling: Supports two-step labeling strategies using amine coupling followed by bioorthogonal tetrazine ligation.
- Nanoparticle Functionalization: Enables modification of appropriately functionalized nanoparticle surfaces and related nanoscale materials.
- Surface Modification: Useful for attaching tetrazine functionality to compatible amine-containing surfaces.
- Biomaterial Research: Supports development of functional materials using amine-reactive and bioorthogonal conjugation chemistry.
Storage and Handling
- Storage: Store according to the product specification in a tightly sealed container under dry conditions. Protect from prolonged light exposure.
- Handling: NHS esters are moisture sensitive and can hydrolyze in aqueous environments. Minimize moisture exposure and prepare aqueous solutions shortly before use.
Methyltetrazine-PEG5-NHS combines amine-reactive NHS ester chemistry with rapid tetrazine ligation through a flexible PEG5 spacer, while its bifunctional design supports sequential labeling, conjugation, and functional material development.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
