NHS-PEG5-tris-alkyne combines an amine-reactive NHS ester, a hydrophilic PEG5 spacer, and three alkyne functionalities in a multifunctional linker. It supports amine labeling, multivalent click chemistry, branched bioconjugation, and multicomponent molecular assembly.
Key Features and Benefits
- Multifunctional Linker: Combines one NHS ester with three alkyne functionalities for sequential and multivalent conjugation strategies.
- NHS Ester Functionality: Reacts with primary amines on proteins, peptides, and other amine-containing molecules.
- PEG5 Spacer: Provides a flexible, hydrophilic spacer that specifically supports molecular accessibility and conjugation applications.
- Three Alkyne Groups: Provides multiple reactive sites for attaching compatible azide-functionalized molecules.
- CuAAC Click Chemistry: Alkyne groups react with azides through copper-catalyzed azide-alkyne cycloaddition.
- Stable Triazole Formation: Alkyne-azide reactions specifically generate stable triazole linkages for constructing molecular conjugates.
- Multivalent Functionalization: Three alkyne groups specifically enable attachment of multiple compatible azide-containing components.
- Sequential Conjugation: NHS chemistry can first modify an amine-bearing molecule, leaving alkyne groups available for subsequent click reactions.
Applications
- Bioconjugation: Supports multistep attachment of biomolecules, probes, ligands, and other functional components.
- Amine Labeling: NHS ester functionality enables modification of primary amines on suitable biomolecules and synthetic compounds.
- Click Chemistry: Multiple alkyne groups support CuAAC reactions with compatible azide-functionalized molecules.
- Multivalent Conjugation: Three alkyne functionalities specifically support attachment of multiple components through one linker architecture.
- Protein Modification: Useful for introducing multiple alkyne reactive sites onto proteins containing accessible primary amines.
- Peptide Modification: Supports amine-directed functionalization of peptides for subsequent azide-alkyne conjugation.
- Nanoparticle Functionalization: Enables multivalent modification of appropriately functionalized nanoparticle systems.
- Biomaterial Research: Supports construction of functional materials using amine-reactive and click-compatible conjugation strategies.
Storage and Handling
- Storage: Store according to the product specification in a tightly sealed container under dry conditions.
- Handling: NHS esters are moisture sensitive and can hydrolyze in aqueous environments. Minimize moisture exposure and prepare aqueous solutions shortly before use.
NHS-PEG5-tris-alkyne combines amine-reactive NHS chemistry with three click-reactive alkyne groups, while its hydrophilic PEG architecture supports sequential conjugation, multivalent functionalization, and complex molecular design.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
