NHS-PEG5-tris-PEG3-aldehyde combines an amine-reactive NHS ester, a hydrophilic PEG5 spacer, and three PEG3-linked aldehyde groups in a multifunctional linker and specifically supports amine labeling, multivalent conjugation, aldehyde-reactive coupling, and branched molecular assembly.
Key Features and Benefits
- Multifunctional Linker: Combines one NHS ester with three aldehyde functionalities specifically for sequential and multivalent conjugation strategies.
- NHS Ester Functionality: Reacts with primary amines on proteins, peptides, and other amine-containing molecules.
- PEG5 Spacer: Provides a flexible, hydrophilic spacer that specifically supports molecular accessibility and conjugation applications.
- Three PEG3-Aldehyde Groups: Provides three PEG3-linked aldehydes for multivalent functionalization with compatible nucleophiles.
- Aldehyde Reactivity: Aldehyde groups specifically support conjugation with hydrazide, aminooxy, and other suitable aldehyde-reactive groups.
- Oxime Formation: Reacts with aminooxy-functionalized molecules to form stable oxime linkages.
- Hydrazone Formation: Supports coupling with hydrazide-containing molecules through hydrazone bond formation.
- Sequential Functionalization: NHS chemistry can first modify an amine-bearing molecule, leaving three aldehydes available for subsequent conjugation.
Applications
- Bioconjugation: Supports multistep attachment of biomolecules, probes, ligands, and other functional components.
- Amine Labeling: NHS ester functionality enables modification of primary amines on suitable biomolecules and synthetic compounds.
- Aldehyde Conjugation: Three aldehyde groups support reactions with aminooxy, hydrazide, and other compatible functionalities.
- Multivalent Conjugation: Multiple aldehyde groups specifically enable attachment of several compatible molecules through one linker architecture.
- Protein Modification: Useful for introducing multiple aldehyde-reactive sites onto proteins containing accessible primary amines.
- Peptide Modification: Supports amine-directed linker attachment followed by aldehyde-based conjugation.
- Nanoparticle Functionalization: Enables multivalent modification of appropriately functionalized nanoparticle systems.
- Biomaterial Research: Supports functional material development using amine-reactive and aldehyde-based conjugation strategies.
Storage and Handling
- Storage: Store according to the product specification in a tightly sealed container under dry conditions.
- Handling: NHS esters are moisture sensitive and can hydrolyze in aqueous environments. Minimize moisture exposure and prepare aqueous solutions shortly before use.
NHS-PEG5-tris-PEG3-aldehyde combines amine-reactive NHS chemistry with three PEG3-linked aldehyde groups, while its branched PEG architecture supports sequential conjugation, multivalent functionalization, and complex molecular design.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
