NHS-PEG5-tris-PEG3-DBCO combines an amine-reactive NHS ester, a hydrophilic PEG5 spacer, and three PEG3-linked DBCO groups in a multifunctional linker and specifically supports amine labeling, multivalent copper-free click chemistry, branched bioconjugation, and multicomponent molecular assembly.
Key Features and Benefits
- Multifunctional Linker: Combines one NHS ester with three DBCO functionalities specifically for sequential and multivalent conjugation strategies.
- NHS Ester Functionality: Reacts with primary amines on proteins, peptides, and other amine-containing molecules.
- PEG5 Spacer: Provides a flexible, hydrophilic spacer that specifically supports molecular accessibility and conjugation applications.
- Three PEG3-DBCO Groups: Provides three PEG3-linked DBCO groups for multivalent functionalization with azide-containing molecules.
- SPAAC Click Chemistry: DBCO groups react efficiently with azides through strain-promoted azide-alkyne cycloaddition.
- Copper-Free Conjugation: Enables azide-DBCO coupling without a copper catalyst for bioorthogonal labeling and conjugation research.
- Stable Triazole Formation: DBCO-azide reactions generate stable triazole linkages specifically for constructing molecular conjugates.
- Sequential Functionalization: NHS chemistry can first modify an amine-bearing molecule, leaving three DBCO groups available for subsequent click reactions.
Applications
- Bioconjugation: Supports multistep attachment of biomolecules, probes, ligands, and other functional components.
- Amine Labeling: NHS ester functionality enables modification of primary amines on suitable biomolecules and synthetic compounds.
- SPAAC Click Chemistry: Three DBCO groups support copper-free conjugation with compatible azide-functionalized molecules.
- Multivalent Conjugation: Multiple DBCO groups enable attachment of several azide-containing molecules through one linker architecture.
- Protein Modification: Useful for introducing multiple DBCO reactive sites onto proteins containing accessible primary amines.
- Peptide Modification: Supports amine-directed linker attachment followed by copper-free click conjugation.
- Nanoparticle Functionalization: Enables multivalent modification of appropriately functionalized nanoparticle systems.
- Biomaterial Research: Supports functional material development using amine-reactive and bioorthogonal conjugation strategies.
Storage and Handling
- Storage: Store according to the product specification in a tightly sealed container under dry conditions. Protect from prolonged light exposure.
- Handling: NHS esters are moisture sensitive and can hydrolyze in aqueous environments. Minimize moisture exposure and prepare aqueous solutions shortly before use.
NHS-PEG5-tris-PEG3-DBCO combines amine-reactive NHS chemistry with three PEG3-linked DBCO groups, while its branched PEG architecture supports sequential conjugation, multivalent SPAAC chemistry, and complex molecular design.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
