Biotin-X-maleimide is a biotinylation reagent designed for labeling molecules containing sulfhydryl groups (-SH), such as cysteine residues in proteins or other thiol-containing compounds. The maleimide group reacts selectively with thiols, forming stable thioether bonds, while the biotin moiety enables strong interaction with avidin or streptavidin.
Key Features:
- Biotinylation: Provides a biotin tag for binding with avidin or streptavidin for detection, purification, or immobilization.
- Selective Reactivity: Maleimide group specifically targets sulfhydryl groups in thiol-containing molecules.
- Spacer Arm: Features an aminocaproic spacer (“X” spacer arm) for improved accessibility and reduced steric hindrance in biotin-streptavidin interactions.
- Applications:
- Protein modification and labeling.
- Affinity purification.
- Detection in immunoassays (e.g., ELISA).
Storage and Handling:
- Store at -20°C in a dry, light-protected environment.
- Use in buffer systems at pH 6.5–7.5 for optimal maleimide reactivity.
Biotin-X-maleimide is a versatile tool for thiol-specific biotinylation in biochemical and molecular biology workflows.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
