DO3A-Thiol provides a thiol-functionalized macrocyclic chelator for bioconjugation, metal coordination, and molecular imaging research and specifically combines the DO3A (1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid) chelating framework with a reactive thiol group. This bifunctional structure specifically supports covalent attachment to suitable biomolecules, polymers, and functionalized surfaces while retaining metal coordination capabilities.
Key Features and Benefits
- DO3A Macrocyclic Framework: Features a tetraazacyclododecane-based chelator with three pendant acetate groups specifically for coordination of compatible metal ions.
- Reactive Thiol Functionality: Provides a sulfhydryl group specifically for covalent conjugation with maleimide-functionalized molecules and other thiol-reactive compounds.
- Bifunctional Chelator Design: Combines metal coordination and bioconjugation capabilities within a single functionalized macrocyclic structure.
- Maleimide-Thiol Coupling Compatibility: Supports formation of covalent thioether-linked conjugates specifically through reactions with suitable maleimide-functionalized molecules.
- Metal Coordination Capability: Enables investigation of DO3A complexation with compatible lanthanide ions and radiometals under optimized conditions.
- Versatile Conjugation Chemistry: Supports thiol-based reactions with maleimides, activated disulfides, and other compatible electrophilic functional groups.
- Biomolecular Functionalization: Enables attachment of DO3A chelators to appropriately functionalized peptides, proteins, antibodies, and targeting ligands.
- Surface Modification Potential: Supports introduction of metal-chelating functionality onto suitable nanoparticles, polymers, and biomaterial surfaces.
Applications
- Thiol-Based Bioconjugation: Supports covalent attachment of DO3A functionality to maleimide-containing biomolecules and synthetic compounds.
- Peptide Modification: Enables preparation of DO3A-functionalized peptides specifically through suitable thiol-reactive conjugation strategies.
- Protein Bioconjugation: Supports introduction of macrocyclic chelators into proteins containing compatible maleimide or other thiol-reactive groups.
- Antibody Functionalization: Useful for developing chelator-modified antibodies through appropriately controlled conjugation reactions.
- Radiopharmaceutical Research: Supports preparation of functionalized chelator conjugates for investigational radiometal labeling applications.
- Molecular Imaging Research: Enables development of metal-chelate molecular probes for diagnostic imaging studies.
- MRI Contrast Agent Research: Supports investigation of DO3A-based lanthanide complexes and their magnetic resonance properties.
- Polymer Functionalization: Useful for incorporating DO3A chelators into maleimide-functionalized polymers, nanoparticles, and biomaterials.
- Coordination Chemistry Studies: Enables investigation of metal-binding behavior, complex stability, and physicochemical properties of functionalized DO3A derivatives.
Storage and Handling
- Storage: Store at −20°C in a dry, tightly sealed container unless otherwise specified. Protect from moisture, excessive heat, and prolonged exposure to air to minimize thiol oxidation.
- Handling: Prepare solutions shortly before use and minimize exposure to oxidizing conditions. For maleimide-thiol conjugation, use suitable mildly acidic to neutral reaction conditions, typically around pH 6.5–7.5. Avoid competing thiol-containing reagents during coupling. Minimize trace-metal contamination when preparing metal complexes.
DO3A-Thiol combines a macrocyclic DO3A chelator with reactive sulfhydryl functionality for versatile biomolecular modification and specifically supports peptide and protein conjugation, radiometal coordination, molecular imaging, and development of functionalized metal-chelate research compounds.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
