5′-ODMT cEt G Phosphoramidite provides a conformationally constrained guanosine building block for modified oligonucleotide synthesis and nucleic acid research and specifically combines a constrained ethyl (cEt) bicyclic sugar framework with a guanine nucleobase, a 5′-O-dimethoxytrityl (DMT) protecting group, and a reactive phosphoramidite functionality. This modified nucleoside specifically supports the development of high-affinity oligonucleotides for antisense, hybridization, and nucleic acid chemistry studies.
Key Features and Benefits
- Constrained Ethyl (cEt) Modification: Features a bicyclic sugar framework designed to restrict nucleoside conformation and enhance complementary RNA binding affinity.
- Guanine Nucleobase: Incorporates a guanine base for sequence-specific recognition of complementary cytosine residues in nucleic acid duplexes.
- 5′-O-DMT Protection: Protects the hydroxyl group during synthesis and specifically supports controlled nucleotide addition through repeated coupling cycles.
- Reactive Phosphoramidite Functionality: Enables incorporation into growing oligonucleotide chains specifically using compatible solid-phase synthesis protocols.
- Enhanced Duplex Affinity: The cEt modification can increase binding affinity toward complementary RNA compared with corresponding unmodified DNA sequences.
- Improved Nuclease Resistance: Supports the development of modified oligonucleotides with increased resistance to enzymatic degradation, depending on sequence and backbone chemistry.
- Conformationally Restricted Sugar: Promotes a defined sugar conformation that specifically influences oligonucleotide hybridization and duplex stability.
- Antisense Oligonucleotide Compatibility: Supports synthesis of cEt-modified antisense sequences, including gapmer designs with appropriately positioned modified nucleotides.
- Versatile Nucleic Acid Modification: Enables investigation of modified nucleotide incorporation, duplex thermodynamics, and oligonucleotide structure-function relationships.
Applications
- Antisense Oligonucleotide Research: Supports development of cEt-modified antisense sequences for investigating RNA recognition and target binding.
- Gapmer Oligonucleotide Synthesis: Enables incorporation of constrained nucleotides into modified flanking regions of antisense gapmer constructs.
- RNA Targeting Research: Supports preparation of oligonucleotides designed to recognize complementary RNA sequences with increased binding affinity.
- Modified Oligonucleotide Synthesis: Useful for introducing cEt guanosine residues into synthetic nucleic acid sequences.
- Nucleic Acid Hybridization Studies: Enables investigation of duplex formation, sequence specificity, and thermal stability in modified oligonucleotides.
- RNase H-Dependent Antisense Research: Supports preparation of gapmer designs containing cEt-modified wings and an RNase H-compatible central DNA region.
- Nuclease Stability Studies: Useful for evaluating the effects of constrained sugar modifications on oligonucleotide degradation and stability.
- Oligonucleotide Structure Research: Supports investigation of sugar conformation, duplex geometry, and molecular recognition.
- Phosphoramidite Chemistry Development: Enables optimization of coupling, deprotection, and purification procedures for constrained nucleoside building blocks.
Storage and Handling
- Storage: Store at −20°C in a dry, tightly sealed container under an inert atmosphere when possible, unless otherwise specified. Protect from moisture, excessive heat, and prolonged light exposure.
- Handling: Handle under anhydrous conditions to preserve phosphoramidite reactivity. Prepare solutions using dry, compatible solvents, such as anhydrous acetonitrile, when appropriate. Minimize atmospheric moisture exposure and use freshly prepared solutions. Apply validated coupling and deprotection conditions compatible with the cEt sugar framework and guanine protecting groups.
5′-ODMT cEt G Phosphoramidite combines a conformationally constrained ethyl sugar modification with a guanine nucleobase for advanced oligonucleotide synthesis and specifically supports antisense gapmer development, RNA targeting research, enhanced hybridization studies, and investigation of modified nucleic acid stability.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
