DMT-dA(PAc) Phosphoramidite provides a protected 2′-deoxyadenosine building block for automated DNA oligonucleotide synthesis and nucleic acid research and specifically combines a 5′-dimethoxytrityl (DMT) protecting group, a phenoxyacetyl (PAc)-protected adenine nucleobase, and a reactive 3′-phosphoramidite functionality. This structure specifically supports controlled incorporation of adenine residues into synthetic DNA sequences using standard phosphoramidite chemistry.
Key Features and Benefits
- Protected Deoxyadenosine Building Block: Features a 2′-deoxyadenosine nucleoside designed for controlled incorporation into synthetic DNA oligonucleotides.
- 5′-DMT Protection: Protects the 5′-hydroxyl group and enables stepwise nucleotide addition during automated solid-phase DNA synthesis.
- Phenoxyacetyl Base Protection: Uses a PAc protecting group on adenine’s exocyclic amino functionality to minimize unwanted side reactions during synthesis.
- Reactive 3′-Phosphoramidite: Supports efficient coupling with growing oligonucleotide chains under suitable activation conditions.
- 2-Cyanoethyl Phosphate Protection: Protects the developing phosphate backbone during synthesis and supports subsequent deprotection.
- Automated Synthesis Compatibility: Supports standard phosphoramidite synthesis cycles using compatible automated DNA synthesizers and validated protocols.
- Controlled Nucleotide Incorporation: Enables sequence-specific addition of adenine residues during stepwise oligonucleotide assembly.
- Deprotection Compatibility: Supports removal of protecting groups under appropriately selected conditions following oligonucleotide synthesis.
- Versatile DNA Synthesis Applications: Supports preparation of primers, probes, modified oligonucleotides, and research-grade DNA sequences.
Applications
- DNA Oligonucleotide Synthesis: Supports automated solid-phase preparation of DNA sequences containing precisely positioned adenine residues.
- PCR Primer Synthesis: Enables preparation of sequence-specific DNA primers for polymerase chain reaction and molecular biology research.
- DNA Probe Development: Supports synthesis of oligonucleotide probes for hybridization, sequence detection, and molecular diagnostic research.
- Modified Oligonucleotide Research: Useful for incorporating protected deoxyadenosine into DNA sequences containing modified nucleotides or functional groups.
- Gene Synthesis Research: Supports preparation of synthetic DNA fragments specifically for sequence assembly and molecular biology applications.
- Antisense Oligonucleotide Research: Enables synthesis of DNA-based sequences for investigating complementary nucleic acid recognition.
- DNA Hybridization Studies: Supports preparation of defined oligonucleotides specifically for investigating duplex formation, sequence specificity, and thermal stability.
- Enzymatic DNA Studies: Useful for preparing substrates specifically for DNA polymerase, nuclease, and other nucleic acid enzyme investigations.
- Nucleic Acid Chemistry: Supports development and optimization of phosphoramidite coupling, protecting group removal, and oligonucleotide purification methods.
Storage and Handling
- Storage: Store at −20°C in a dry, tightly sealed container under an inert atmosphere when possible, unless otherwise specified. Protect from moisture, excessive heat, and prolonged light exposure.
- Handling: Handle under anhydrous conditions to preserve phosphoramidite reactivity. Prepare solutions using dry, compatible solvents, such as anhydrous acetonitrile, when appropriate. Minimize exposure to atmospheric moisture and use freshly prepared solutions for oligonucleotide synthesis. Select coupling and deprotection conditions compatible with the phenoxyacetyl protecting group.
DMT-dA(PAc) Phosphoramidite combines protected deoxyadenosine with reactive phosphoramidite functionality for controlled DNA synthesis and specifically supports oligonucleotide assembly, PCR primer preparation, DNA probe development, nucleic acid chemistry, and molecular biology research.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
