Fmoc-Ala-Ala-Asn(Trt)-PAB-PNP
This product is a high-purity, cleavable peptide linker commonly used in specifically antibody-drug conjugate (ADC) development. It features an Fmoc-protected Ala-Ala-Asn(Trt) tripeptide sequence linked to a p-aminobenzyl (PAB) self-immolative spacer and a p-nitrophenyl carbonate (PNP) reactive group. As a result, this design allows for controlled, enzyme-triggered release of payloads in targeted drug delivery systems.
Key Features and Benefits:
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ADC-Validated Linker Design: Incorporates an Ala-Ala-Asn(Trt) peptide sequence for selective enzymatic cleavage.
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PAB Self-Immolative Spacer: Ensures efficient and complete payload release after cleavage.
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PNP Reactive Group: Provides an activated carbonate for rapid and efficient coupling with hydroxyl or amine-containing drugs.
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Fmoc and Trt Protection: Maintains orthogonal reactivity, allowing stepwise synthesis and functionalization.
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High Purity: Supplied at >95% purity for reproducibility in research and preclinical development.
Applications:
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Antibody-Drug Conjugates (ADCs): Enables selective release of cytotoxic payloads in target cells.
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Targeted Drug Delivery: Facilitates enzyme-cleavable drug-linker systems for precision therapeutics.
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Bioconjugation: Couples with drugs, peptides, or biomolecules in multistep synthesis workflows.
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Prodrug Development: Incorporates into cleavable prodrug systems for controlled activation.
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Chemical Biology Research: Used in studying enzyme-triggered release mechanisms.
Storage and Handling:
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Storage: Store at -20°C, in a dry, dark environment.
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Handling: Use anhydrous solvents such as DMSO or DMF; avoid prolonged exposure to moisture to maintain PNP activity.
This product combines enzymatic cleavability, self-immolative release, and orthogonal reactivity, making it an especially powerful tool for ADC linker design and advanced drug delivery applications.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
