TODi-3 (Thiazole Red Homodimer, TOTO®-3, CAS 166196-17-4)
TODi-3, also known as Thiazole Red Homodimer or TOTO®-3, is a far-red fluorescent dye. It binds strongly to double-stranded DNA (dsDNA) and RNA (dsRNA). Once bound, it becomes highly fluorescent, which makes it ideal for sensitive nucleic acid staining.
Key Features:
- Fluorescence Properties:
- Excitation: ~642 nm
- Emission: ~660 nm
- Produces bright far-red fluorescence when bound.
- High Sensitivity: It detects low amounts of nucleic acids with minimal background fluorescence.
- Non-Permeant: Since it does not enter live cells, it is perfect for staining dead or fixed cells.
- Far-Red Spectrum: Works well in multicolor experiments, with little interference from other dyes.
Applications:
- Nucleic Acid Detection: It stains dsDNA and dsRNA in microscopy and flow cytometry.
- Cell Viability Tests: It distinguishes dead cells by staining nucleic acids in compromised cells.
- Chromosome and Nuclear Staining: It provides clear nuclear staining for multicolor imaging.
- Gel Electrophoresis: It visualizes nucleic acids in agarose or polyacrylamide gels.
- Microarrays: It enables high-sensitivity detection in high-throughput studies.
Handling and Storage:
- Form: Provided in a DMSO solution.
- Storage: Store at -20°C, protected from light.
- Precautions: Handle with protective equipment in a ventilated space.
In summary, TODi-3 is a trusted dye for far-red fluorescence. It offers strong nucleic acid binding, high sensitivity, and excellent compatibility with various applications.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
