TODi-1 (Thiazole Orange Homodimer, TOTO®-1, CAS 143413-84-7)
TODi-1, also known as Thiazole Orange Homodimer or TOTO®-1, is a green fluorescent dye. Specifically, it binds strongly to double-stranded DNA (dsDNA) and RNA (dsRNA). Upon binding, it becomes highly fluorescent, which makes it particularly ideal for sensitive nucleic acid detection.
Key Features:
- Fluorescence:
- Excitation Maximum: ~514 nm
- Emission Maximum: ~533 nm
- Strong fluorescence enhancement upon binding to nucleic acids.
- High Sensitivity: Detects low levels of dsDNA and dsRNA with minimal background fluorescence.
- Non-Permeant: Does not penetrate live cell membranes, ideal for distinguishing live and dead cells.
TODi-1 Applications:
- Nucleic Acid Detection:
- Stains dsDNA and dsRNA in fluorescence microscopy and flow cytometry.
- Cell Viability Assays:
- Differentiates live cells from dead cells by staining nucleic acids in compromised cells.
- Chromosome and Nuclear Staining:
- Visualizes nuclear structures in multicolor fluorescence studies.
- Gel Electrophoresis:
- Detects nucleic acids in agarose or polyacrylamide gels.
- Microarrays:
- Stains nucleic acids in high-throughput applications.
Handling and Storage:
- Form: Provided as a DMSO solution.
- Storage: Store at -20°C, protected from light.
- Precautions: Handle with proper protective equipment in a well-ventilated area.
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