TO-TAP-1 (Thiazole Orange Monomer, TO-PRO®-1, CAS 157199-59-2)
TO-TAP-1 is a green fluorescent carbocyanine dye that binds nucleic acids, becoming highly fluorescent upon binding to double-stranded DNA (dsDNA). It is widely used in molecular biology for nucleic acid staining and cell viability assays.
Key Features:
- Fluorescence Properties:
- Excitation Maximum: ~513 nm
- Emission Maximum: ~533 nm
- Nonfluorescent in the absence of nucleic acids.
- Selective Binding: Binds strongly to dsDNA, with enhanced fluorescence upon binding.
- Cell Impermeant: Ideal for distinguishing between live and dead cells.
- Storage: Typically provided as a DMSO solution; store at -20°C, protected from light.
Applications:
- Nucleic Acid Staining:
- Visualizes dsDNA in fluorescence microscopy and flow cytometry.
- Cell Viability Assays:
- Differentiates dead cells in live/dead viability studies.
- Chromosome and Nuclear Counterstaining:
- Provides clear nuclear staining in multicolor fluorescence labeling experiments.
- Microarray Studies:
- Stains nucleic acids on microarrays for high-sensitivity detection.
- Quantitative DNA Analysis:
- Enables precise DNA quantification in biological samples.
TO-TAP-1 is a reliable tool for nucleic acid research, offering high specificity, bright fluorescence, and versatility across various molecular and cellular biology applications.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
