Thiol-PEG-OH, MW 5K
Thiol-PEG-OH (Molecular Weight: 5,000 Da) is a monofunctional polyethylene glycol derivative with a thiol group (-SH) on one end and a hydroxyl group (-OH) on the other. The thiol group enables selective conjugation to maleimide-, vinyl sulfone-, iodoacetyl-, or gold-surface-functionalized molecules, while the hydroxyl terminus offers stability or potential for further modification. The PEG backbone improves aqueous solubility, reduces nonspecific interactions, and enhances biocompatibility in both research and biomedical applications.
Key Features and Benefits:
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Thiol Functional Group (-SH): Reacts selectively with maleimide, vinyl sulfone, iodoacetyl, or gold surfaces for stable covalent or coordinate bonds.
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Hydroxyl Terminus (-OH): Provides stability and can be derivatized for secondary functionalization.
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PEG Backbone (MW 5K): Improves water solubility, increases hydrophilicity, and reduces protein adsorption.
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High Purity: Supplied at >95% purity for reproducible and consistent performance.
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Surface Passivation: PEG coating minimizes nonspecific binding and reduces immunogenicity.
Applications:
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Nanoparticle Coating: Functionalizes gold nanoparticles, quantum dots, and biosensor surfaces via thiol-gold chemistry.
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Protein Conjugation: Couples to maleimide- or iodoacetyl-containing proteins, peptides, or enzymes.
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Drug Delivery Systems: PEGylates liposomes, micelles, or polymeric nanoparticles to extend circulation time.
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Biosensor and Diagnostic Development: Immobilizes biomolecules on thiol-reactive surfaces.
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Hydrogel and Polymer Modification: Enhances hydrophilicity and biointerface control.
Storage and Handling:
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Storage: Store at -20°C, in a dry environment protected from light.
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Handling: Use under inert gas (nitrogen or argon) to prevent thiol oxidation. Dissolve in water or compatible organic solvents such as DMSO or DMF before use.
Thiol-PEG-OH, MW 5K provides a reliable combination of thiol-specific reactivity and PEG-mediated solubility, making it an ideal choice for nanoparticle functionalization, bioconjugation, and surface passivation applications.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
