TCO-PEG5-DBCO
This product is a heterobifunctional linker containing a trans-cyclooctene (TCO) group, a PEG5 spacer, and a dibenzocyclooctyne (DBCO) group. The TCO reacts ultrafast with tetrazines via copper-free IEDDA ligation, while the DBCO undergoes SPAAC click chemistry with azides. The PEG5 chain improves solubility, provides moderate spacing, and reduces steric hindrance, making this reagent especially effective for dual-functional conjugation strategies.
Key Features and Benefits
-
TCO Group: Enables ultrafast, copper-free IEDDA ligation with tetrazines.
-
DBCO Group: Performs strain-promoted click chemistry with azides.
-
PEG5 Spacer: Adds solubility, flexibility, and accessibility.
-
Dual Reactivity: Offers two orthogonal click handles in one molecule.
-
High Purity: Manufactured to ≥95% purity for reproducibility.
Applications
-
Bioconjugation: Links tetrazine- and azide-modified biomolecules.
-
Protein and Antibody Labeling: Provides dual orthogonal handles for multifunctional modifications.
-
Probe Development: Builds dual-reactive probes for diagnostics and imaging.
-
Drug Delivery: Serves as a modular linker for therapeutic conjugates.
Storage and Handling
-
Storage: Store at –20 °C in a sealed, desiccated container.
-
Handling: Prepare fresh solutions; avoid repeated freeze–thaw cycles.
This product integrates ultrafast tetrazine ligation with SPAAC click chemistry in a PEG5 framework, making it an especially versatile linker for advanced conjugation.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
