t-Boc-N-Amido-PEG4-Azide

t-Boc-N-Amido-PEG4-Azide

t-Boc-N-Amido-PEG4-Azide is a monodisperse, heterobifunctional PEG linker containing a Boc-protected amine and a terminal azide group. Moreover, the PEG4 spacer offers enhanced aqueous solubility, reduced steric hindrance, and optimal flexibility for a variety of bioorthogonal conjugation strategies. As a result, this reagent is ideal for applications involving click chemistry and stepwise bioconjugation.

Key Features and Benefits:

• Azide Functional Group: Reacts efficiently with alkynes via copper-catalyzed (CuAAC) or copper-free (SPAAC) click chemistry.

• Boc-Protected Amine: Allows selective deprotection under mild acidic conditions for downstream amide bond formation.

• PEG4 Spacer: Provides hydrophilicity and flexibility while minimizing immunogenicity and nonspecific interactions.

• Orthogonal Reactivity: Enables sequential conjugation of biomolecules, drug carriers, or surfaces with high precision.

• High Purity: Supplied at >95% purity for reliable, reproducible performance in advanced bioconjugation workflows.

Applications:

• Click Chemistry Conjugation: Attach to DBCO or alkyne-containing molecules for modular assembly of bioconjugates.

• Stepwise Bioconjugation: Use the Boc-protected amine for second-stage reactions after click chemistry labeling.

• Protein and Peptide Modification: Introduce azide handles for site-specific labeling or tracking.

• Nanoparticle and Surface Functionalization: Modify hydrogels, liposomes, or polymer surfaces with azide groups.

• Drug Delivery and Diagnostic Design: Develop dual-functional linkers for targeted therapies and biosensor development.

Storage and Handling:

• Storage: Store at -20°C, in a dry environment protected from light.

• Handling: Deprotect the Boc group using 50% TFA in DCM or equivalent. Use dry solvents such as DMSO or DMF to maintain reactivity.

t-Boc-N-Amido-PEG4-Azide delivers reliable performance for researchers designing orthogonal bioconjugates, click chemistry platforms, and PEG-modified biomolecules. As a result, its compact PEG4 chain balances solubility with controlled spacing, making it a valuable tool in modern chemical biology.

AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.