t-Boc-Aminooxy-PEG4-Tos is a crosslinker containing a t-Boc-aminooxy group and a tosyl group. The protected aminooxy can be deprotected under mild acidic conditions to form free aminooxy. The aminooxy has been used to conjugate with carbonyl bearing (ketone and aldehyde) biomolecules to form oxime for reversible linkage spacer.Oxime ligation is one of the hot topics in the field of bioconjugation of molecules due to its chemoselectivity and hydrolytic stability. The PEG spacer increases the aqueous solubility of the resulting compound. The tosyl group is a very good leaving group for nucleophilic substitutions.
Axispharm offers 3000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.