Sulfo-NThiol-SS-biotin
Sulfo-NThiol-SS-biotin is a high-purity, water-soluble biotinylation reagent with a cleavable disulfide bond and a terminal thiol group specifically for selective coupling to maleimides, haloacetyls, or other thiol-reactive species. The disulfide linkage enables controlled release under mild reducing conditions, while the sulfonated structure ensures excellent aqueous solubility specifically for efficient bioconjugation without organic co-solvents.
Key Features and Benefits
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Terminal Thiol Group – Reacts selectively with maleimides, haloacetyls, disulfides, and gold surfaces
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Cleavable Disulfide Bond – Allows controlled release of biotinylated molecules under reducing conditions
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Biotin Moiety – Enables strong, specific binding to streptavidin and avidin conjugates
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Sulfonated Structure – Provides high water solubility, eliminating the need for organic solvents
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High Purity – Supplied at >95% purity for reliable and reproducible performance
Applications
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Reversible protein and antibody biotinylation
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Thiol-specific labeling in aqueous environments
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Controlled-release affinity tagging
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Biosensor and diagnostic assay development
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Surface and nanoparticle functionalization
Storage and Handling
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Store at –20 °C, dry, and protected from light
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Handling: Use freshly prepared aqueous solutions for optimal reactivity and avoid prolonged exposure to oxidizing agents to maintain thiol activity
This product is a water-soluble, cleavable biotinylation reagent with a thiol for selective coupling and a disulfide bond for controlled release, especially ideal for reversible and high-specificity labeling in fully aqueous systems.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
