Sulfo-Cyanine5 Streptavidin is a conjugate of streptavidin, a high-affinity biotin-binding protein, and sulfo-Cyanine5, a far-red fluorescent dye. It is designed for sensitive detection of biotin-labeled molecules with minimal nonspecific binding and high photostability.
Key Features:
- Streptavidin Properties:
- Tetrameric protein derived from Streptomyces avidinii, capable of binding up to four biotin molecules.
- High affinity and selectivity due to hydrogen bonds and van der Waals interactions.
- Minimal nonspecific binding compared to avidin, with high resistance to extreme pH, heat, and enzymatic degradation.
- Fluorescence Properties:
- Sulfo-Cyanine5 is hydrophilic, with excitation ~646 nm and emission ~662 nm.
- Far-red fluorescence reduces background autofluorescence, ideal for imaging.
- Applications:
- Immunofluorescent staining, Western blotting, flow cytometry, and microplate assays.
- Specific detection of biotin-labeled proteins, nucleic acids, lipids, and other biomolecules.
Usage Guidelines:
- Concentration Range: 0.5–10 µg/mL.
- Avoid biotin-containing solutions (e.g., RPMI 1640, some serums) to prevent interference.
Storage and Handling:
- Store at -20°C, protected from light.
- Use freshly prepared solutions for optimal results.
Sulfo-Cyanine5 Streptavidin offers reliable and versatile detection for various biotin-based experimental workflows.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
