Sulfo-Cyanine5 NHS Ester (CAS: 2230212-27-6) is a water-soluble, far-red fluorescent dye commonly used for labeling amine-containing biomolecules, such as proteins and peptides. Its sulfonated structure enhances hydrophilicity, allowing efficient conjugation in aqueous environments without the need for organic solvents.
Key Features:
- Excitation/Emission Maxima: Approximately 646 nm/662 nm, suitable for use with standard Cy5 filter sets.
- High Water Solubility: Facilitates labeling in purely aqueous solutions, preserving the native state of sensitive biomolecules.
- Amine Reactivity: The NHS ester group specifically reacts with primary amines, forming stable amide bonds for reliable conjugation.
Applications:
- Fluorescence Microscopy: Ideal for imaging applications requiring far-red emission.
- Flow Cytometry: Suitable for multi-color analysis due to minimal spectral overlap.
- Protein and Peptide Labeling: Enables the study of biomolecular interactions and localization.
Handling and Storage:
- Molecular Weight: Approximately 777.95 g/mol.
- Storage Conditions: Store at -20°C, protected from light and moisture to maintain stability.
This dye works as a direct replacement for Cy5®, Alexa Fluor® 647, and DyLight® 649 in various fluorescence-based applications.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
