Sulfo-Cyanine3 Tyramide is a water-soluble fluorescent tyramide reagent (excitation ~550 nm, emission ~570 nm) used in Tyramide Signal Amplification (TSA), a highly effective method for enhancing fluorescence intensity in immunohistochemistry (IHC), immunocytochemistry (ICC), and fluorescence in situ hybridization (FISH).
Key Features:
- TSA Method: Utilizes horseradish peroxidase (HRP) and hydrogen peroxide to convert labeled tyramides into reactive intermediates that covalently bind to tyrosine residues near the target site. This increases sensitivity by over 100-fold compared to conventional methods.
- Signal Enhancement: Ideal for detecting low-abundance targets or reducing antibody/probe concentrations while maintaining strong signal intensity and reducing background staining.
- Multiplexing Capability: Covalent binding enables sequential labeling with multiple dyes for multiplexed imaging.
- Applications: IHC, ICC, FISH, and fluorescence microscopy.
Storage and Handling:
- Store at -20°C, protected from light.
- Compatible with various TSA kits and protocols.
This tyramide provides high sensitivity and flexibility for advanced fluorescence-based detection.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
