PLLA-PEG-FITC, MW x 4K, n 5K provides a fluorescently labeled, biodegradable amphiphilic block copolymer for nanoparticle imaging, drug delivery, and biomaterials research and specifically combines a 4 kDa poly(L-lactic acid) (PLLA) segment with a 5 kDa hydrophilic polyethylene glycol (PEG) chain functionalized with fluorescein isothiocyanate (FITC). Its PEG-rich composition specifically supports polymer self-assembly, fluorescent nanoparticle tracking, and cellular uptake studies.
Key Features and Benefits
- 4 kDa PLLA Block: Provides a biodegradable hydrophobic polyester segment specifically for nanoparticle formation, drug encapsulation, and controlled-release research.
- 5 kDa PEG Block: Introduces a hydrophilic polymer chain that specifically supports aqueous dispersion, surface hydration, and colloidal stabilization.
- FITC Fluorescent Label: Incorporates a fluorescein-derived fluorescent group for tracking polymeric materials using compatible fluorescence detection systems.
- Green Fluorescence Detection: Supports fluorescence microscopy and analytical measurements using excitation and emission settings appropriate for FITC.
- Amphiphilic Block Copolymer: Combines hydrophobic PLLA and hydrophilic PEG segments to specifically support self-assembly into polymeric nanoparticles and related nanostructures.
- Biodegradable Polyester Segment: Contains hydrolytically degradable ester bonds for investigating polymer degradation and controlled-release behavior.
- PEG-Rich Composition: Features a nominal 4:5 PLLA-to-PEG molecular weight ratio specifically for studying hydrophilic-hydrophobic balance, polymer assembly, and nanoparticle stability.
- Fluorescent Polymer Tracking: Enables visualization and quantitative analysis of labeled polymer distributions in compatible experimental systems.
- Hydrophilic Surface Functionality: Provides a 5 kDa PEG segment for investigating nanoparticle hydration, surface interactions, and fluorescence accessibility.
Applications
- Fluorescent Nanoparticle Imaging: Supports visualization of FITC-labeled polymeric nanoparticles using fluorescence microscopy and related imaging techniques.
- Cellular Uptake Studies: Enables investigation of nanoparticle association and internalization in cultured cells through fluorescence-based detection.
- Drug Delivery Research: Supports development and characterization of fluorescent polymeric carriers for drug encapsulation and delivery studies.
- Polymeric Nanoparticle Development: Enables preparation of biodegradable nanoparticles containing hydrophobic PLLA domains and hydrophilic PEG surfaces.
- Polymeric Micelle Research: Supports investigation of amphiphilic self-assembly, micelle formation, and fluorescent nanocarrier stability.
- Nanoparticle Distribution Studies: Enables fluorescence-based tracking of labeled polymer distribution in suitable experimental models.
- Controlled Drug Release: Supports evaluation of drug release behavior alongside fluorescence-based monitoring of polymeric carrier distribution.
- Biomaterials Research: Enables development of fluorescent biodegradable polymer systems for investigating material interactions and cellular responses.
- Polymer Characterization: Supports analysis of nanoparticle size, fluorescence intensity, polymer composition, colloidal stability, and degradation behavior.
Storage and Handling
- Storage: Store at −20°C in a dry, tightly sealed container, protected from light and moisture, unless otherwise specified. Minimize exposure to excessive heat and prolonged illumination to preserve polymer integrity and FITC fluorescence.
- Handling: Handle under dry conditions and protect from direct light during preparation and analysis. Dissolve in a compatible organic solvent, such as DMSO or DMF, after confirming product-specific solubility. For nanoparticle preparation, select an appropriate nanoprecipitation, solvent exchange, or emulsification method based on polymer properties and formulation requirements.
- For fluorescence studies, use compatible FITC excitation and emission settings, typically near 495 nm and 520 nm, respectively. Actual fluorescence characteristics may vary with the conjugate and experimental environment. Maintain consistent sample concentrations, minimize photobleaching, and account for the pH sensitivity of fluorescein fluorescence. Avoid prolonged exposure to strongly acidic or alkaline conditions that may accelerate PLLA degradation.
PLLA-PEG-FITC, MW x 4K, n 5K combines biodegradable PLLA, hydrophilic PEG, and FITC fluorescence for versatile nanocarrier development and imaging research and specifically supports fluorescent nanoparticle tracking, cellular uptake studies, drug delivery research, polymeric micelle development, and biomaterials characterization.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.