Norbornene-PEG11-Biotin
This product is a heterobifunctional linker that integrates a norbornene group, an eleven-unit PEG spacer, and a biotin tag. The norbornene moiety enables rapid bioorthogonal conjugation through inverse electron-demand Diels–Alder (IEDDA) ligation with tetrazines, while the biotin provides strong affinity binding to streptavidin, avidin, and NeutrAvidin. The PEG11 chain enhances solubility, flexibility, and biotin accessibility, making this reagent especially effective for bioconjugation, affinity assays, and probe development.
Key Features and Benefits
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Norbornene Group: Supports ultrafast IEDDA ligation with tetrazines.
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Biotin Tag: Ensures high-affinity binding to streptavidin/avidin systems.
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PEG11 Spacer: Improves solubility and reduces steric hindrance.
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Dual Reactivity: Combines bioorthogonal conjugation with biotin-based affinity capture.
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High Purity: Produced to ≥95% purity for reproducibility.
Applications
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Affinity Capture: Enables efficient immobilization on streptavidin-coated beads, chips, or surfaces.
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Bioconjugation: Links biomolecules with tetrazines and captures them via biotin–streptavidin binding.
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Probe Development: Creates dual-functional probes for imaging and diagnostics.
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Drug Delivery: Serves as a biotinylated, bioorthogonal linker for targeted systems.
Storage and Handling
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Storage: Store at –20 °C in a sealed, light- and moisture-protected container.
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Handling: Prepare fresh solutions; avoid repeated freeze–thaw cycles.
This product combines rapid click chemistry with strong biotin–streptavidin affinity on a PEG11 backbone, specifically offering a versatile reagent for advanced conjugation and assay development.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
