N,N-Bis(PEG1-azide)-N-PEG2-NHS ester
N,N-Bis(PEG1-azide)-N-PEG2-NHS ester is a high-purity trifunctional PEG linker featuring two azide groups for bioorthogonal click chemistry (CuAAC or SPAAC) and an NHS ester for rapid amine coupling. The PEG1 and PEG2 spacers improve aqueous solubility, enhance flexibility, and reduce steric hindrance, enabling efficient multi-point bioconjugation in both aqueous and organic systems. This unique design supports the creation of multifunctional conjugates for advanced drug delivery, diagnostics, and material science applications.
Key Features and Benefits
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Dual Azide Groups: Allow simultaneous or sequential click chemistry reactions with alkyne-functionalized molecules.
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NHS Ester: Reacts efficiently with primary amines under mild conditions to form stable amide bonds.
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PEG1 + PEG2 Spacers: Enhance solubility, flexibility, and accessibility of reactive groups.
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Trifunctional Design: Enables multi-point conjugation for complex bioconjugates.
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High Purity: Supplied at >95% purity for reproducible, high-yield results.
Applications
- Multifunctional Bioconjugation: Create branched or multi-label constructs.
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Protein and Peptide Modification: Attach two alkyne-containing molecules while coupling to an amine site.
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Nanoparticle Functionalization: Introduce multiple reactive handles for targeted surface engineering.
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Drug Delivery Systems: Build multifunctional linkers enabling precise payload attachment and release.
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Diagnostic Probe Development: Construct multi-label imaging or sensing agents.
Storage and Handling
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Storage: Store at –20 °C, dry, and protected from light.
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Handling: Use anhydrous solvents such as DMF or DMSO; avoid moisture to preserve NHS ester and azide reactivity.
N,N-Bis(PEG1-azide)-N-PEG2-NHS ester offers two azide click handles and an amine-reactive NHS ester in a highly soluble, flexible PEG framework, making it an especially powerful tool for multifunctional bioconjugates, targeted delivery, and diagnostic applications.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
