Methyltetrazine-SS-PEG4-Biotin is a cleavable, multifunctional linker combining methyltetrazine for bioorthogonal click chemistry, a disulfide bond for controlled cleavage under reducing conditions, and biotin for strong affinity to streptavidin or avidin. The PEG4 spacer ensures solubility and reduces steric hindrance.
Key Features
- Methyltetrazine Group
Facilitates rapid and selective IEDDA reactions with strained alkenes (e.g., trans-cyclooctene). - Disulfide Bond
Provides cleavable functionality for controlled release in reducing environments. - Biotin Tag
Enables high-affinity binding to streptavidin or avidin for enrichment or detection. - PEG4 Spacer
Enhances solubility and minimizes steric effects during conjugation.
Applications
- Bioconjugation
Enables site-specific labeling of biomolecules with bioorthogonal and biotin functionality. - Affinity-Based Applications
Useful in pull-down assays, purification, or detection of biotinylated molecules. - Controlled Drug Delivery
Supports cleavable conjugates for targeted release. - Molecular Probes
Ideal for designing imaging or diagnostic agents requiring dual functionalities.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
