Mal-PEG4-(PEG3-DBCO)-(PEG3-TCO) is a versatile, tri-functional PEG linker designed for advanced bioconjugation and chemical biology. It contains:
- A maleimide group for thiol-specific conjugation.
- A DBCO (dibenzocyclooctyne) group for copper-free azide-alkyne cycloaddition (SPAAC).
- A TCO (trans-cyclooctene) group for rapid, bioorthogonal ligation with tetrazines.
The PEG4 and PEG3 linkers improve solubility, flexibility, and reduce steric hindrance, making it suitable for a wide range of biological and chemical applications.
Key Features
- Functional Groups:
- Maleimide: Forms stable bonds with thiol groups (-SH).
- DBCO: Enables copper-free reactions with azides for selective conjugation.
- TCO: Reacts quickly and selectively with tetrazines using IEDDA chemistry.
- PEG Linkers:
- Enhance solubility and flexibility.
- Minimize steric hindrance for efficient conjugation in aqueous environments.
- Biocompatibility:
- Non-toxic and suitable for both in vivo and in vitro use.
Applications
- Bioconjugation: Attaches biomolecules like peptides, proteins, or nucleic acids through thiol, azide, and tetrazine chemistry.
- Drug Delivery: Serves as a multifunctional linker to enhance solubility and targeting in drug conjugates.
- Nanotechnology: Functionalizes nanoparticles, biosensors, and surfaces for dual bioorthogonal labeling.
- Chemical Biology: Enables copper-free labeling, dual modifications, and crosslinking in live-cell and in vivo systems.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
