Mal-amido-PEG7-NHS Ester
Mal-amido-PEG7-NHS Ester acts as a bifunctional linker designed for bioconjugation and molecular research. Its structure includes a maleimide group that specifically reacts with thiol groups (-SH), forming stable thioether bonds. Simultaneously, its NHS ester efficiently binds to primary amines, creating robust amide bonds. The seven-unit PEG spacer improves water solubility and molecular flexibility, reducing steric hindrance during conjugation.
Key Features:
- Reactive Groups:
- Maleimide: Selectively couples with thiol-containing molecules such as cysteine residues in proteins, enabling precise molecular linking.
- NHS Ester: Forms strong amide bonds by reacting with primary amines found in lysine residues or protein N-termini.
- PEG7 Spacer:
- Increases solubility in aqueous environments, making the linker suitable for biological systems.
- Provides molecular flexibility while minimizing steric interference, enhancing conjugation efficiency.
Applications:
- Bioconjugation:
- Attaches biomolecules such as peptides, proteins, and antibodies by coupling thiol and amine-reactive groups.
- PROTAC Synthesis:
- Functions as a crucial linker in proteolysis-targeting chimeras (PROTACs), supporting protein degradation via the ubiquitin-proteasome pathway.
Handling and Storage:
- Molecular Formula: C₂₈H₄₃N₃O₁₄
- Molecular Weight: 645.65 g/mol
- Storage Recommendations:
- Store at 2-8°C in a dry, dark environment, away from light and moisture, to preserve its chemical stability.
Mal-amido-PEG7-NHS Ester enables efficient and precise bioconjugation due to its dual reactivity and extended PEG7 spacer. Its unique properties make it indispensable for chemical biology, drug development, and advanced therapeutic research.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
