Mal-amido-PEG6-NHS ester
Mal-amido-PEG6-NHS ester is a high-purity heterobifunctional PEG linker with a maleimide group for thiol-specific conjugation and an NHS ester for rapid amine coupling. The PEG6 spacer provides excellent water solubility, flexibility, and reduced steric hindrance, enabling efficient bioconjugation in both aqueous and organic systems. This design is ideal for orthogonal conjugation strategies where thiol and amine chemistries are used sequentially.
Key Features and Benefits
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Maleimide Group: Selectively reacts with thiols to form stable thioether bonds.
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NHS Ester: Couples efficiently with primary amines under mild conditions to form stable amide bonds.
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PEG6 Spacer: Enhances solubility, flexibility, and accessibility of reactive groups.
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Heterobifunctional Design: Supports orthogonal and sequential modification strategies.
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High Purity: Supplied at >95% purity for reproducible and consistent results.
Applications
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Stepwise Bioconjugation: Attach thiols first, then couple to amines, or vice versa.
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Protein and Peptide Modification: Introduce thiol-reactive and amine-reactive handles in one linker.
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Nanoparticle and Surface Functionalization: Functionalize surfaces with dual reactivity for complex assemblies.
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Drug Delivery Systems: Build multifunctional linkers for targeted therapeutic delivery.
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Diagnostic and Imaging Probes: Create dual-labeled probes with orthogonal chemistries.
Storage and Handling
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Storage: Store at –20 °C, dry, and protected from light.
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Handling: Use anhydrous solvents such as DMF or DMSO; avoid prolonged moisture exposure to preserve NHS ester reactivity.
Mal-amido-PEG6-NHS ester combines thiol and amine conjugation chemistries in a hydrophilic PEG6 framework, offering high solubility and versatility for orthogonal bioconjugation in therapeutics, diagnostics, and materials science.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
