m-PEG-GAS, MW 2K provides an activated polyethylene glycol derivative for protein PEGylation, bioconjugation, and drug delivery research and specifically combines a 2 kDa methoxy-terminated polyethylene glycol (mPEG) chain with a glutaric acid N-hydroxysuccinimide ester (GAS) reactive group. This amine-reactive polymer specifically supports covalent attachment to proteins, peptides, and other amino-functionalized molecules through stable amide bond formation.
Key Features and Benefits
- 2 kDa PEG Chain: Features a methoxy-terminated polyethylene glycol chain with a nominal molecular weight of 2,000 Da for bioconjugation research.
- Activated GAS Functionality: Contains a glutaric acid-derived N-hydroxysuccinimide ester for efficient reaction with primary amines.
- Amine-Reactive PEGylation: Supports covalent modification of lysine residues, N-terminal amino groups, and other accessible primary amines.
- Stable Amide Bond Formation: Forms covalent amide linkages through reactions between the activated ester and suitable amino groups.
- Methoxy-Terminated Structure: Provides a nonreactive methoxy terminus that supports single-ended PEG conjugation.
- Hydrophilic PEG Backbone: Introduces a water-compatible polymer segment for investigating solubility, hydration, and molecular interactions.
- Short PEG Chain: The 2 kDa PEG segment enables studies of PEGylation effects while introducing less polymer mass than higher-molecular-weight alternatives.
- Flexible Glutaric Acid Spacer: Provides a glutaric acid-derived linker between the PEG chain and the conjugated molecule.
- Versatile Bioconjugation: Enables attachment of PEG chains to peptides, proteins, enzymes, and other amine-containing research compounds.
Applications
- Protein PEGylation: Supports covalent attachment of 2 kDa PEG chains to accessible protein amino groups for stability and solubility studies.
- Peptide Modification: Enables PEGylation of peptides containing suitable primary amines for investigating physicochemical properties.
- Enzyme PEGylation: Useful for evaluating the effects of PEG modification on enzyme stability, activity, and aggregation.
- Drug Delivery Research: Supports preparation of PEGylated molecular constructs for investigating drug delivery and formulation properties.
- Bioconjugation Research: Enables NHS ester-mediated coupling of PEG to amine-functionalized biomolecules and synthetic compounds.
- Protein Solubility Studies: Supports investigation of PEGylation effects on protein solubility, aggregation, and solution behavior.
- Biomaterial Functionalization: Useful for modifying amine-containing polymers, hydrogels, and other compatible biomaterial structures.
- PEGylated Conjugate Development: Supports preparation of PEG-modified peptides, proteins, and research compounds for molecular characterization.
- Polymer Characterization: Enables investigation of PEG molecular weight, conjugation efficiency, and physicochemical properties of PEGylated products.
Storage and Handling
- Storage: Store at −20°C in a dry, tightly sealed container under an inert atmosphere when possible, unless otherwise specified. Protect from moisture and excessive heat to minimize hydrolysis of the activated ester.
- Handling: Handle under dry conditions and prepare solutions immediately before use. For PEGylation reactions, dissolve the reagent in a compatible anhydrous solvent or suitable reaction buffer. Use amine-free buffers, such as phosphate buffer, typically around pH 7.0–8.5. Avoid Tris and other primary amine-containing buffers that compete with the target molecule. Minimize prolonged exposure to aqueous solutions because the NHS ester undergoes hydrolysis.
m-PEG-GAS, MW 2K combines a hydrophilic 2 kDa PEG chain with an amine-reactive glutaric acid NHS ester for efficient bioconjugation and specifically supports protein and peptide PEGylation, enzyme modification, drug delivery research, biomaterial functionalization, and development of PEGylated molecular constructs.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
