Fluorescein-DBCO (CAS: 2054339-00-1) is a fluorescent dye conjugate combining fluorescein with a dibenzocyclooctyne moiety. It enables copper-free click chemistry through strain-promoted alkyne-azide cycloaddition (SPAAC), making it ideal for labeling azide-containing biomolecules without the need for a copper catalyst.
Key Features:
- Functional Groups: DBCO for SPAAC reactions and fluorescein for fluorescence detection.
- Molecular Formula: C₃₉H₂₇N₃O₆S
- Molecular Weight: 665.71 g/mol
- Excitation Maximum: 494 nm
- Emission Maximum: 517 nm
Fluorescein-DBCO Applications:
- Fluorescent Labeling: Tags azide-functionalized biomolecules for imaging and detection.
- Bioconjugation: Forms stable triazole linkages with azide-bearing compounds in biochemical assays.
- Live-Cell Imaging: Suitable for labeling live cells due to copper-free compatibility, reducing cytotoxicity.
- Antibody-Drug Conjugates (ADCs): Acts as a non-cleavable linker for targeted drug delivery research.
This compound is highly effective for applications requiring fast, specific, and copper-free conjugation, making it ideal for live-cell imaging and sensitive biological assays.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
