endo-BCN-PEG2-Biotin
endo-BCN-PEG2-Biotin is a high-purity heterobifunctional linker combining an endo-bicyclo[6.1.0]non-4-yne (endo-BCN) group for rapid, catalyst-free strain-promoted azide–alkyne cycloaddition (SPAAC) with a biotin moiety for strong and specific binding to streptavidin or avidin. The PEG2 spacer enhances solubility, flexibility, and reduces steric hindrance, ensuring efficient conjugation and optimal biotin–protein interaction in both aqueous and organic systems. This design is especially ideal for bioorthogonal labeling, affinity purification, and biotin-based detection assays.
Key Features and Benefits
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Endo-BCN Group: Enables fast, copper-free SPAAC click chemistry with azides, preserving biomolecule integrity.
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Biotin Moiety: Provides high-affinity binding to streptavidin and avidin for capture, immobilization, or detection.
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PEG2 Spacer: Improves solubility and molecular flexibility, reducing steric interference in conjugation and binding.
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Heterobifunctional Design: Allows orthogonal, site-specific modifications in complex biomolecular assemblies.
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High Purity: Supplied at >95% purity for reliable and reproducible results.
Applications
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Copper-free bioorthogonal labeling of azide-modified biomolecules.
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Streptavidin-based affinity purification and pull-down assays.
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Biotinylation of proteins, peptides, nucleic acids, and nanoparticles.
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Biosensor surface functionalization.
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Imaging and diagnostic assay development.
Storage and Handling
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Storage: Store at –20 °C, dry, and protected from light.
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Handling: Use anhydrous solvents such as DMF or DMSO; avoid moisture to preserve BCN reactivity.
This product combines a copper-free click handle with a biotin targeting group via a PEG2 spacer, specifically delivering high solubility, flexibility, and specificity for labeling, detection, and purification applications.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
