DusQ 21 NHS Ester (CAS 304014-13-9) is a non-fluorescent dark quencher designed for fluorescence resonance energy transfer (FRET) applications. It features an N-hydroxysuccinimide (NHS) ester functional group, enabling efficient conjugation to primary amines in biomolecules such as proteins, peptides, and oligonucleotides.
Key Features:
- Absorption Maximum: 656 nm
- Extinction Coefficient: 72,800 L·mol⁻¹·cm⁻¹
- Quenching Range: 590–720 nm
Applications:
- FRET Assays: Serves as an acceptor for fluorophores emitting in the red to near-infrared spectrum, including Cyanine5, Cyanine5.5, and Alexa Fluor 647.
- Multiplex Assays: Ideal for complex assays due to its lack of secondary fluorescence.
- Bioconjugation: Efficiently labels amine-containing biomolecules for various analytical and diagnostic purposes.
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