DTSSP Crosslinker
This product is a water-soluble, cleavable crosslinker containing two NHS esters and a central disulfide bond. It reacts with primary amines to form amide bonds, and its disulfide linkage specifically allows reversible cleavage under reducing conditions.
Key Features and Benefits:
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Water-Soluble: Eliminates need for organic solvents in biological applications.
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Dual NHS Esters: Rapidly couple to amines on proteins, peptides, or surfaces.
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Cleavable Disulfide Bond: Enables reversible crosslinking via mild reducing agents.
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High Specificity: Provides targeted conjugation to primary amines.
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Versatile Use: Suitable for structural biology, protein interaction studies, and assay development.
Applications:
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Protein Crosslinking: Links lysine residues for interaction and structural analysis.
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Reversible Conjugation: Facilitates temporary labeling or modification with cleavable bonds.
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Bioconjugation: Builds stable but cleavable constructs for controlled studies.
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Cell Biology: Supports crosslinking in aqueous, physiological environments.
Storage and Handling:
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Storage: Store at –20 °C in a desiccated, light-protected environment.
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Handling: Dissolve freshly in aqueous buffers before use; avoid repeated freeze–thaw cycles.
This product offers efficient, water-soluble, and reversible amine-to-amine conjugation, making it an especially powerful tool for protein interaction mapping and reversible labeling.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.