DSPE-Polysarcosine-Azide, DP 25 is an azide-functionalized lipid-polymer conjugate featuring a hydrophobic DSPE lipid anchor and hydrophilic polysarcosine chain. The polysarcosine segment has approximately 25 repeat units, while the terminal azide supports bioorthogonal click chemistry and bioconjugation.
Key Features and Benefits
- Amphiphilic Structure: DSPE-Polysarcosine-Azide combines a hydrophobic phospholipid anchor with a hydrophilic polysarcosine chain.
- Polysarcosine DP 25: The polymer segment contains approximately 25 sarcosine repeat units, providing a compact hydrophilic spacer.
- DSPE Lipid Anchor: DSPE contains two saturated C18 stearoyl chains that support incorporation into lipid membranes and assemblies.
- Azide Functionality: The terminal azide (-N₃) provides a bioorthogonal reactive site specifically for click chemistry and conjugation.
- CuAAC Click Chemistry: The azide reacts with terminal alkynes specifically through copper-catalyzed azide-alkyne cycloaddition.
- SPAAC Click Chemistry: The azide reacts with strained cyclooctynes, such as DBCO, without requiring a copper catalyst.
- Hydrophilic Polymer Spacer: Polysarcosine provides hydrophilic separation between the lipid anchor and reactive azide functionality.
- Functional Lipid-Polymer Platform: DSPE, polysarcosine, and azide functionality provide an especially versatile platform for lipid and nanomaterial modification.
Applications
- Click Chemistry: DSPE-Polysarcosine-Azide, DP 25 supports CuAAC and SPAAC reactions with compatible alkyne and cyclooctyne molecules.
- Bioconjugation: The terminal azide enables attachment of compatible ligands, peptides, proteins, probes, and other functional molecules.
- Lipid Nanoparticle Research: Supports incorporation of azide-functionalized polysarcosine into experimental lipid nanoparticle systems.
- Liposome Research: Enables functionalization of liposomes and related lipid assemblies with hydrophilic polysarcosine chains.
- Ligand Conjugation: Supports attachment of alkyne- or cyclooctyne-functionalized ligands through click chemistry.
- Nanoparticle Functionalization: Provides a click-reactive polymer terminus for modification of compatible nanoparticle systems.
- Surface Modification: Supports presentation of hydrophilic polysarcosine chains and reactive azide groups on compatible surfaces.
- Biomaterial Research: Enables development of functional lipid-polymer materials for conjugation and biomaterial studies.
Storage and Handling
- Storage: Store at –20 °C in a tightly sealed container under dry conditions.
- Handling: Protect from moisture and excessive heat. Allow the material to reach room temperature before opening to minimize condensation.
DSPE-Polysarcosine-Azide, DP 25 combines a DSPE lipid anchor, compact polysarcosine chain, and click-reactive terminal azide and specifically supports click chemistry, lipid formulation, bioconjugation, surface modification, and biomaterial research.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.