DOTA-PEG4-azide hydrochloride provides an azide-functionalized macrocyclic chelator for click chemistry, bioconjugation, and radiopharmaceutical research and specifically combines a DOTA chelating framework with a hydrophilic tetraethylene glycol (PEG4) spacer and a reactive azide group. This bifunctional hydrochloride salt specifically supports attachment to alkyne-containing biomolecules and preparation of metal-chelate conjugates for molecular imaging research.
Key Features and Benefits
- DOTA Macrocyclic Chelator: Features a tetraazacyclododecane-based chelator with four acetate arms specifically for coordination of compatible metal ions.
- Reactive Azide Functionality: Provides an azide group for selective conjugation with terminal or strained alkyne-functionalized molecules.
- Hydrophilic PEG4 Spacer: Incorporates a tetraethylene glycol linker to specifically provide flexibility and improve hydrophilic character between the chelator and conjugation site.
- Click Chemistry Compatibility: Supports copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) for forming stable triazole-linked molecular conjugates.
- Copper-Free Conjugation: Enables strain-promoted azide-alkyne cycloaddition (SPAAC) with compatible strained alkynes, including DBCO derivatives.
- Bifunctional Chelator Design: Combines metal coordination and bioorthogonal conjugation capabilities within a single molecular structure.
- Hydrochloride Salt Form: Provides the compound in hydrochloride form specifically for research applications requiring a salt-form chelator derivative.
- Radiometal Coordination Potential: Supports investigation of complexation with compatible radiometals, including gallium-68, lutetium-177, and indium-111.
- Versatile Biomolecular Modification: Enables attachment of DOTA functionality to appropriately modified peptides, proteins, antibodies, and targeting ligands.
Applications
- Click Chemistry Bioconjugation: Supports CuAAC-mediated attachment of DOTA chelators to terminal alkyne-functionalized biomolecules and synthetic compounds.
- Copper-Free Click Chemistry: Enables SPAAC conjugation with DBCO-functionalized peptides, proteins, and other compatible molecules.
- Radiopharmaceutical Research: Supports preparation of DOTA-functionalized molecular constructs for investigational radiometal labeling applications.
- Peptide Functionalization: Enables conjugation of DOTA-PEG4 functionality to alkyne-modified peptides for molecular imaging research.
- Protein Bioconjugation: Supports attachment of macrocyclic chelators to proteins containing appropriately incorporated alkyne groups.
- Antibody Conjugation: Useful for developing chelator-functionalized antibodies through compatible bioorthogonal conjugation strategies.
- PET Imaging Research: Supports development of investigational radiometal-labeled probes for positron emission tomography.
- SPECT Imaging Research: Enables preparation of DOTA-containing molecular probes for single-photon emission computed tomography studies.
- Targeted Radionuclide Research: Supports development of targeting ligand conjugates for investigating radiometal coordination and radionuclide delivery.
Storage and Handling
- Storage: Store at −20°C in a dry, tightly sealed container unless otherwise specified. Protect from moisture, excessive heat, and prolonged light exposure.
- Handling: Handle using appropriate laboratory precautions and minimize trace-metal contamination. For CuAAC reactions, use suitable copper catalysts, ligands, and terminal alkyne-functionalized reaction partners. For copper-free conjugation, use compatible strained alkynes such as DBCO. Remove residual copper when necessary before radiometal labeling or biological studies. Optimize reaction conditions according to the target molecule and intended application.
DOTA-PEG4-azide hydrochloride combines a DOTA macrocyclic chelator with a hydrophilic PEG4 spacer and reactive azide functionality for versatile bioconjugation and specifically supports click chemistry, peptide and antibody modification, radiometal labeling, molecular imaging, and targeted radionuclide research.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
