DBCO-PEG3-SS-NHS is a cleavable, heterobifunctional linker widely used in bioconjugation. It combines a DBCO group for copper-free click chemistry with azides and an NHS ester that reacts with primary amines to form stable amide bonds. The PEG3 spacer improves solubility and reduces steric hindrance, while the disulfide bond enables cleavage under reducing conditions, allowing for controlled release of conjugated components.
Key Features:
- DBCO Group: Allows for strain-promoted azide-alkyne cycloaddition (SPAAC) without copper, enhancing biocompatibility.
- NHS Ester: Facilitates conjugation with proteins, peptides, or other amine-containing molecules.
- PEG3 Spacer: Increases solubility and flexibility, minimizing steric hindrance.
- Disulfide Bond: Allows cleavage under reducing conditions for controlled release.
Applications:
- Antibody-Drug Conjugates (ADCs): Attaches cytotoxic drugs to antibodies for targeted cancer therapy.
- Protein Labeling: Enables site-specific labeling for imaging or tracking.
- Biomolecule Immobilization: Attaches biomolecules to surfaces or other molecules for biosensors and diagnostic assays.
Store DBCO-PEG3-SS-NHS at -20°C, and dissolve it in solvents like DCM, THF, acetonitrile, DMF, or DMSO before use.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
