Cyanine7 NHS ester

Cyanine7 (Cy7) NHS Ester: Overview and Applications

Cyanine7 (Cy7) NHS Ester is an amine-reactive, near-infrared fluorescent dye, serving as an improved analog of Cy7®. Its rigidified polymethyne chain increases quantum yield by 20%, enhancing fluorescence brightness.

Key Features:

• Fluorescent Properties: Emits NIR light (750-800 nm), taking advantage of the near-infrared window for effective in vivo imaging.
• NHS Ester Reactivity: Reacts with primary amines at physiological pH, forming stable amide bonds for efficient conjugation.
• High Sensitivity: Provides bright fluorescence for sensitive detection in assays.

Applications:

• Protein Labeling: Labels proteins and antibodies for enhanced detection in biological studies.
• Fluorescence Imaging: Ideal for in vivo imaging, delivering high-resolution images with low background noise.
• Flow Cytometry: Tags cells for accurate analysis and sorting.
• Bioconjugation: Produces Cyanine7-labeled biomolecules for in vivo research and drug design.

Cyanine7 NHS Ester’s strong fluorescence and efficient coupling make it a valuable tool in molecular biology, diagnostics, and drug development.