Cyanine3.5 is a bright and photostable fluorophore with spectral characteristics similar to Cy3.5 ™, Alexa Fluor ™ 594 and Alexa Fluor ™ 568. Cyanine3.5 DBCO fluorescence has a maximum fluorescence of 604 nm and is located in the orange-red spectral region.
Cyanine3.5 fluorescence is insensitive to pH from 4 to 10 and is detected in the low autofluorescence region of biological samples.
Cyanine3.5 DBCO (dibenzocyclooctyne) easily forms conjugates with azide derivatives of biomolecules in copper-free Click Chemistry reactions. The process takes place in the absence of catalysts or at elevated temperatures, leading to the formation of stable triazoles.
The conjugates of biomolecules with Cyanine3.5 can be used for various studies using microscopy, including FRET microscopy, as well as in proteomic studies.
AxisPharm offers 4000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.