Cy5 DBCO NHS Ester

Cy5 DBCO NHS Ester: Premium Fluorescent Dye for Advanced Labeling

Product Overview

Cy5 DBCO NHS Ester is a high-performance fluorescent dye designed for precise and efficient labeling in biological research. Featuring the Cy5 dye with an NHS ester group and a dibenzocyclooctyne (DBCO) reactive moiety, this product excels in applications requiring strong fluorescence and specific conjugation.

Key Features

• Bright Fluorescence: Cy5 provides intense and stable fluorescence in the far-red spectrum (around 647-665 nm). Its absorption and emission spectra are almost identical to those of Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes, ideal for detailed imaging and multi-color experiments.
• DBCO Reactivity: The dibenzocyclooctyne (DBCO) group enables copper-free click chemistry for clean and efficient bioconjugation.
• NHS Ester Group: The N-hydroxysuccinimide (NHS) ester ensures rapid and effective coupling with amine-containing biomolecules, making it a versatile choice for various labeling needs.

Applications

1. Fluorescence Microscopy: Ideal for high-resolution imaging of cellular and molecular structures.
2. Flow Cytometry: Useful for detailed analysis of cell populations and interactions.
3. Bioconjugation: Facilitates the creation of stable conjugates with proteins, peptides, and other biomolecules via click chemistry.

Benefits

• Efficient Labeling: The combination of Cy5, DBCO, and NHS ester groups provides a robust solution for precise and reliable labeling.
• Minimal Background: The far-red fluorescence minimizes overlap with other dyes, enhancing signal clarity in multi-color assays.
• Versatile Use: Suitable for a wide range of applications including microscopy, flow cytometry, and protein labeling.

Conclusion

Cy5 DBCO NHS Ester is an essential tool for researchers seeking high-quality fluorescent labeling. Its advanced features, including bright far-red fluorescence, efficient bioconjugation capabilities, and versatility, make it an ideal choice for applications in imaging, flow cytometry, and molecular studies. Enhance your research with this premium fluorescent dye for clear, reliable, and precise results.

Ref:

https://www.thermofisher.com/us/en/home/life-science/cell-analysis/fluorophores/cy5-dye.html

H.A. Shindy, Fundamentals in the chemistry of cyanine dyes: A review, Dyes and Pigments, Volume 145, 2017, Pages 505-513, ISSN 0143-7208.

AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.