Cy3 DBCO is a bright, far-red-fluorescent, probe routinely used for imaging of azide-containing biomolecules without the need for copper catalyst. Cy3 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern Cy3 DBCO is an ideal alternative to copper requiring fluorescent alkynes.
Cy3 is a bright, water-soluble, and pH-insensitive orange-fluorescent dye that can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Its absorption and emission spectra are almost identical to those of Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine5-based fluorescent dyes.
Cy®3 DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.
This is sulfonated dye is also known as sulfo-Cyanine3.
Cy®Dye is a trademark of GE Healthcare. Alexa Fluor® 555 is a registered trademark of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.