Boc-Aminooxyacetamide-PEG2-azide

Boc-Aminooxyacetamide-PEG2-azide

Boc-Aminooxyacetamide-PEG2-azide is a versatile heterobifunctional PEG linker featuring an azide group for click chemistry and a protected aminooxy group for oxime ligation. The PEG2 spacer improves solubility and provides flexibility between functional groups. This reagent is ideal for dual-site bioconjugation, especially when combining copper-free click reactions with ketone or aldehyde coupling strategies.

Key Features and Benefits:

• Click-Ready Azide Group: Compatible with copper-catalyzed (CuAAC) and copper-free strain-promoted click chemistry (SPAAC).

• Boc-Protected Aminooxy Group: Can be deprotected under mild acidic conditions to reveal a reactive aminooxy group for oxime bond formation with carbonyl-containing biomolecules.

• PEG2 Spacer: Enhances solubility and reduces steric hindrance, while maintaining short, controlled linker length.

• Heterobifunctional Design: Enables sequential or orthogonal conjugation for complex bioconjugate construction.

• High Purity: Supplied at >95% purity to ensure reliable and reproducible results in sensitive applications.

Applications:

• Bioconjugation: Combine azide-alkyne click chemistry with oxime ligation to create multifunctional biomolecules.

• Protein and Glycoprotein Labeling: Modify aldehyde- or ketone-tagged proteins using the aminooxy group.

• Nanoparticle Functionalization: Introduce dual reactivity for controlled surface modification.

• Diagnostic Assay Development: Build bifunctional probes or reporter systems with precise control.

• Chemical Biology and Drug Discovery: Synthesize branched or modular molecules for structure-activity studies.

Storage and Handling:

• Storage: Store at -20°C in a dry, dark environment.

• Handling: Deprotect the Boc group using mild acid (e.g., 50% TFA in DCM) just before use. Use anhydrous solvents (e.g., DMSO, DMF) for best reactivity.

Boc-Aminooxyacetamide-PEG2-azide provides a powerful dual-functional linker for modern bioconjugation strategies. Additionally, with orthogonal reactivity and excellent solubility, it supports precise and modular conjugate design for advanced research and therapeutic applications.

AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.