t-Boc-Aminooxy-PEG4-acid linker contains a t-Boc-protected Aminooxy group and a terminal carboxylic acid. The terminal carboxylic acid readily reacts with primary and secondary amine under EDC, DCC, HATU or other amide coupling conditions to form a stable amide bond. The PEG spacer increases the aqueous solubility of the resulting compound. The t-Boc-protected Aminooxy can be deprotected under mild acidic conditions.The Aminooxy has been used to conjugate with carbonyl bearing (ketone and aldehyde) biomolecules to form oxime for reversible linkage spacer.Oxime ligation is one of the hot topics in the field of bioconjugation of molecules due to its chemoselectivity and hydrolytic stability.
AxisPharm offers 4000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.