t-Boc-Aminooxy-PEG4-acid
t-Boc-Aminooxy-PEG4-acid is a high-purity heterobifunctional PEG linker with a Boc-protected aminooxy group for controlled carbonyl coupling and a terminal carboxylic acid for amide bond formation. The PEG4 spacer improves solubility, flexibility, and reduces steric hindrance, enabling efficient bioconjugation in both aqueous and organic systems. The Boc protection keeps the aminooxy group inert until mild acidic deprotection, allowing orthogonal, stepwise conjugation strategies.
Key Features and Benefits
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Boc-Protected Aminooxy: Stable under neutral and basic conditions; deprotects under mild acidic conditions to couple with aldehydes or ketones via oxime formation.
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Carboxylic Acid: Activatable via carbodiimide or active ester chemistry for amide bond formation.
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PEG4 Spacer: Enhances solubility, flexibility, and accessibility of reactive groups.
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Heterobifunctional Design: Enables orthogonal and sequential modification strategies.
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High Purity: Supplied at >95% purity for reproducible and consistent results.
Applications
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Stepwise Bioconjugation: Protect the aminooxy group until the desired stage for carbonyl coupling.
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Protein and Peptide Modification: Introduce aminooxy functionality after acid-mediated deprotection.
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Nanoparticle and Surface Functionalization: Add dual carbonyl- and amide-reactive sites to materials.
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Drug Delivery Systems: Build multifunctional linkers for sequential payload attachment.
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Diagnostic and Imaging Probes: Prepare orthogonally reactive probes for complex labeling strategies.
Storage and Handling
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Storage: Store at –20 °C, dry, and protected from light.
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Handling: Use anhydrous solvents such as DMF or DMSO; avoid prolonged exposure to moisture to maintain reactivity.
t-Boc-Aminooxy-PEG4-acid combines a protected aminooxy group with a carboxylic acid in a hydrophilic PEG4 framework, offering flexibility for orthogonal bioconjugation in therapeutics, diagnostics, and materials science.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
