Azido-PEG3-NHS Ester (CAS: 1245718-89-1)
Azido-PEG3-NHS Ester is a versatile reagent used in bioconjugation and click chemistry applications. It features an azide group and an NHS ester linked through a three-unit polyethylene glycol (PEG) spacer, enhancing solubility and reactivity.
Key Features:
- Azide Group: Enables copper-catalyzed azide-alkyne cycloaddition (CuAAC) and strain-promoted alkyne-azide cycloaddition (SPAAC) reactions, facilitating stable triazole linkage formation with alkyne, BCN, and DBCO.
- NHS Ester: Reacts efficiently with primary amines (-NH₂) on proteins, peptides, and other biomolecules, allowing for site-specific conjugation.
- PEG Spacer: The hydrophilic PEG chain increases aqueous solubility and reduces steric hindrance, improving conjugation efficiency.
Applications:
- Antibody-Drug Conjugates (ADCs): To link cytotoxic agents to antibodies, such as in the synthesis of ADCs like Cantuzumab mertansine and Cantuzumab ravtansine.
- Protein Labeling: Facilitates the attachment of fluorescent dyes or other probes to proteins for detection and analysis.
- Biomolecule Conjugation: Enables the attachment of various molecules to biomolecules for research and therapeutic purposes.
Handling and Storage:
- Storage: Store at -20°C, protected from light and moisture.
- Handling: Use standard laboratory precautions when handling this reagent.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
