APDye Fluor 647-PEG4-TCO is a far-red fluorescent dye with excitation and emission maxima at 648 nm and 671 nm, similar to Alexa Fluor® 647 and Cy5. It features a PEG4 linker for improved solubility and reduced steric hindrance, along with a trans-cyclooctene (TCO) group for fast, specific bioorthogonal conjugation through tetrazine-mediated click chemistry.
Key Features:
- Bright Far-Red Fluorescence: Compatible with 633 nm and 647 nm laser lines
- Water-Soluble and pH-Stable: Works well across a pH range of 4 to 10
- PEG4 Linker: Enhances solubility and minimizes steric hindrance
- TCO Functional Group: Enables fast and copper-free click chemistry with tetrazine-containing compounds
Applications:
- Fluorescence Microscopy: High-resolution cellular and biomolecular imaging
- Flow Cytometry: Suitable for precise cell analysis
- Bioconjugation: Efficient labeling of proteins, antibodies, and other biomolecules
Handling and Storage:
- Molecular Weight: ~1295.5 g/mol
- Purity: ≥95%
- Storage: Store at -20°C, protected from light and moisture
APDye Fluor 647-PEG-TCO is a powerful tool for advanced biological research, offering reliable fluorescence, efficient conjugation, and broad compatibility with fluorescence-based assays.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
