Ald-benzyl-PEG4-CH2 tBu-ester
Ald-benzyl-PEG4-CH2 tBu-ester is a high-purity heterobifunctional PEG linker with an aldehyde-functionalized benzyl group for chemoselective oxime or hydrazone ligation and a t-butyl ester-protected carboxylic acid for controlled activation. The PEG4 spacer enhances water solubility, flexibility, and reduces steric hindrance, enabling efficient bioconjugation in both aqueous and organic systems. The t-butyl ester group keeps the carboxylic acid inert until mild acidic deprotection, allowing stepwise conjugation strategies.
Key Features and Benefits
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Aldehyde-Benzyl Group: Reacts with aminooxy or hydrazide partners to form stable oxime or hydrazone bonds under mild conditions.
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t-Butyl Ester-Protected Carboxylic Acid: Stable under neutral and basic conditions; easily deprotected under acidic conditions to reveal a reactive acid.
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PEG4 Spacer: Improves solubility, flexibility, and accessibility of reactive groups.
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Heterobifunctional Design: Supports orthogonal, sequential modification strategies.
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High Purity: Supplied at >95% purity for consistent and reproducible results.
Applications
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Stepwise Bioconjugation: Protect the acid until a later synthesis stage for controlled activation.
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Protein and Peptide Modification: Attach aldehyde-reactive groups before acid deprotection.
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Nanoparticle and Surface Functionalization: Introduce aldehyde and protected acid groups for dual reactivity.
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Drug Delivery Systems: Build multifunctional linkers for sequential payload attachment.
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Diagnostic and Imaging Probes: Enable orthogonal modifications for dual-labeled probe synthesis.
Storage and Handling
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Storage: Store at –20 °C, dry, and protected from light.
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Handling: Use anhydrous solvents such as DMF or DMSO; avoid prolonged moisture exposure to maintain reactivity.
This product combines aldehyde-mediated conjugation with a protected acid group in a hydrophilic PEG4 framework, offering versatility for orthogonal bioconjugation in therapeutics, diagnostics, and materials science.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
