Tetrazines demonstrate exceptionally fast cycloaddition kinetics (up to 30 000 M-1 s-1) with trans-cyclooctenes (TCO) as the dienophile, the fastest kinetics ever reported for any bioorthogonal reaction. In addition, inverse-Electron-Demand Diels-Alder reaction of tetrazines trans-cyclooctenea forms a stable covalent bond and does not require Cu-catalyst or elevated temperatures.
APDye 660R is a bright and photostable far-red dye that emits fluorescence at about 685 nm in the borderline spectral region between far-red and near-IR. Although the absorption maximum is at around 665 nm, this dye can be sufficiently excited by the 633 or 635 nm laser. APDye 660R dye is water soluble and pH-insensitive from pH 4 to pH 10. APDye 660R is a rhodamine-based dye, and like rhodamine dyes in general, it is exceptionally photostable (Figure 1). The superior photostability and excellent brightness of APDye 660R make the dye an ideal choice for confocal microscopy and other demanding applications.
APDye 660R dye spectrally is almost identical to Alexa Fluor® 660 and CF® 660R Dye and can be used a less expensive alternative to these dyes.
Alexa Fluor® is a registered trademark of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium.
Axispharm offers 3000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.