AFDye 488 Alkyne (CAS: 1006592-47-7)
AFDye 488 Alkyne is a green-fluorescent dye, equivalent to Alexa Fluor® 488, featuring an alkyne group for click chemistry. It reacts with azide-containing molecules through copper-catalyzed azide-alkyne cycloaddition (CuAAC), forming stable triazole linkages. This dye is ideal for imaging low-abundance azide-tagged biomolecules.
Key Features:
- Bright Fluorescence: Excites at 493 nm and emits at 518 nm, providing strong green signals for imaging.
- Click Chemistry Compatibility: The alkyne group enables efficient conjugation with azide-tagged molecules.
- High Photostability: Maintains fluorescence over time, making it suitable for extended imaging.
Applications:
- Imaging: Detects low-abundance azide-tagged biomolecules in fluorescence microscopy.
- Flow Cytometry: Labels cells for analysis with bright and stable fluorescence.
- Bioconjugation: Attaches to azide-modified biomolecules for research applications.
Handling and Storage:
- Storage: Store at -20°C, away from light and moisture.
- Handling: Follow standard laboratory precautions when using this reagent.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
