2′-OMe-G(iBu) Phosphoramidite
2′-OMe-G(iBu) Phosphoramidite is a high-purity guanosine phosphoramidite with a 2′-O-methyl modification for enhanced nuclease resistance and binding affinity, and an N²-isobutyryl (iBu) protecting group for efficient synthesis. The cyanoethyl (CE) phosphoramidite group enables rapid, efficient solid-phase oligonucleotide assembly, while the 5′-O-dimethoxytrityl (DMT) group allows controlled stepwise chain elongation. This design is especially valuable for producing stable, high-performance RNA oligonucleotides for research, diagnostics, and therapeutic applications.
Key Features and Benefits
- 2′-O-Methyl Modification: Enhances nuclease resistance, increases duplex stability, and improves hybridization affinity.
- N²-Isobutyryl Protection: Minimizes side reactions during synthesis and ensures clean deprotection.
- CE-Phosphoramidite Group: Compatible with standard RNA solid-phase synthesis protocols.
- 5′-O-DMT Protection: Supports stepwise synthesis with high coupling efficiency.
- High Purity: Supplied at ≥95% purity (up to ≥99% for therapeutic grade) for reproducible and reliable results.
Applications
- Nuclease-Resistant Oligonucleotides: Synthesize RNA sequences with improved stability.
- Antisense and siRNA Development: Enhance efficacy and longevity of gene-silencing molecules.
- Aptamer and Ribozyme Synthesis: Create functional RNA with greater durability.
- Diagnostic Probes: Develop stable probes for qPCR, FISH, and hybridization assays.
- Therapeutic Oligonucleotides: Manufacture high-purity RNA sequences for clinical use.
Storage and Handling
- Storage: Store at –20 °C, dry, and under inert atmosphere.
- Handling: Use anhydrous acetonitrile or similar dry solvents; avoid moisture to maintain phosphoramidite reactivity.
2′-OMe-G(iBu) Phosphoramidite combines 2′-O-methyl stability enhancement with efficient guanosine protection and CE-phosphoramidite chemistry, enabling especially high-fidelity RNA synthesis for applications in therapeutics, diagnostics, and molecular biology.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
